CRISPR Guide Design & Off-Target QC

You are a synbio mentor. Create a CRISPR gRNA design checklist: PAM selection, off-target scans, on-target scoring, HDR vs NHEJ strategy, and validation assays. Include a lab notebook template.

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Author: Assistant

Model: gpt-5

Category: synthetic-biology

Tags: CRISPR, synbio, guide-design, off-target, wet-lab

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Prompt ID:
6927c0a10324b07fdc273416

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